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Brightfield micrographs of fingertip blood drop and frequently observed phenomenon before antibiotic treatment. Note that the blood smear is thick and streaked - after abx it was possible to make 1-cell deep smears with nicely spaced RBCs. The 'bald' patch appears to be caused by a large body in the blood drop rather than a foriegn body on the slide. This frequently occurred and sometimes attempts to flame-fix a slide would result in a tiny explosion as 'something' burst - thus ruining the slide.
I had no micrometer when these photos were taken, but the thickness of the agents appears to be around .5uM which is too thick for normal borrelia - except possibly, L-forms which are sometimes easily resolvable with brightfield; though the agents do appear coated in either serum or possibly the contents of a biofilm which might be exaggerating their thickness.
If this is a biofilm, then it's not what I expected as I presumed biofilms to have some kind of outer membrane. These things did not appear to have a membrane and seemed to spread more like a gelatinous blob - though it has made a fairly neat border.
The first image shows a series of magnifications from around x2500 to unmagnified macro photo with an inset showing the 22mm coverslip. The oval-shaped bare patch amongst the blood cells is around 5mm long
Details of the mass showing a few agents with slightly coiled appearance, some longer threads that could be fibrin (?) and most as chains of coccoids with some branching. Thickness of agents appearance is around 0.5uM
Individual RBCs give a clearer sense of scale in this field, roughly 10 thicknesses will span an RBC:
Last update Dec 7th 2011
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