Borrelia FITC stain experiment

These pictures are from a thin-film blood smear stained with FITC. This is KPL product: 02-97-91:

"Affinity purified polyclonal antibody to Borrelia burgdorferi made in Goat and labeled with fluorescein isothiocyanate (FITC). Isolated from a serum pool of goats immunized with heat killed whole cells of Borrelia burgdorferi. The antibody is highly specific for Borrelia burgdorferi." (Link to product page)

The large image in the following sets of micrographs show the subject in a 400x400 pixel selection from the original widefield view (1600x1200 pixels) at a magnification of 100 x 15 x camera zoom @ 35mm. The small images are same with camera zoom @ 105mm. These were taken using long-exposures of between 3 and 10 seconds which eliminates noise. The second small picture (with the red square) is the close-up processed using 'Focus Magic' V3.02 by Acclaim Software (www.focusmagic.com).

Heavy focus processing can produce misleading anomalies which is why I include the original for fair assessment. Some of the subjects appear to be long agents, coiled, folded and even exhibiting 'string of pearl' growth. If these are l-form spirochetes and some are inside erythrocytes it raises questions of whether their presence is harmful and how they got there.

 

Last update July 9th 2012

Navigate to:

BSK Culture Experiment Introduction
BSK Culture Experiment Page 2
BSK Culture Experiment Page 3
BSK Culture Experiment Page 4
BSK Culture Experiment Page 5 Looking at coccoids attached to spiral agents
BSK Culture Experiment Page 6 Attempt at rapid culture - spiral forms produced in a week to 10 days
Biofilms Page 7 A phenomenon pre- or early treatment possibly following long infection
BSK Culture Experiment Page 8 FITC staining borrelia specific
BSK Culture Experiment Page 9 FITC staining borrelia specific high magnification and image processing
L-form Videos Blood and blood+BSK short culture time
Romanowsky Stain Thin film periferal blood smears stained with 'Diff-Quik' and viewed under darkfield