Borrelia Garinii 3 FITC

Venous buffy coat blood sample was stained with Abcam's Anti-Borrelia burgdorferi garinii antibody (FITC) [ab20118 abcam.com].

Method: One drop of fluorescent antibody stain (1:20 distilled water) on centre of slide. Added small drop of blood from buffy coat and mixed. 50mm coverslip with grease on each corner placed and pushed down until whole area covered with sample. Stored in light-proof container with small vial of water to reduce dessication for 24 hours. CONTROL: as above with distilled water instead of stain. Long sides of coverslip sealed with adhesive before microscopy.

Note the diffuse tiny bodies that are fluorescing strongly given their small size. These are probably 'propagules' - granular forms of borrelia capable of growing into spirochetes in suitable conditions.

Matched pairs of darkfield image on the left and fluorescence filtered on the right. Images edited only for noise and size.

Following images are from a control slide with distilled water instead of stain. They demonstrate minimal autofluorescence of lymphocytes using this method. They are deliberately overexposed to show background fluorescence levels.

Last update January 31st 2014

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