Borrelia Garinii 4 FITC

Some fresh fingertip blood was stained with Abcam's Anti-Borrelia burgdorferi garinii antibody (FITC) [ab20118].

Method: One drop of fluorescent antibody stain (1:20 distilled water) on centre of slide. Added small drop of fingertip blood and mixed. 50mm coverslip with grease on each corner placed and pushed down until whole area covered with sample. Stored in light-proof container with small vial of water to reduce dessication for 26 hours. CONTROL (image 3): as above with distilled water instead of stain. Long sides of coverslip sealed with adhesive before microscopy. Donor NHS negative for borreliosis.

Matched pairs of darkfield and fluorescence filtered images edited only for noise and size. This slide shows what happens when a large biofilm in the sample ruptures under the coverslip, spreading spirochetes and granular forms widely. When studying the pictures, please note how often very bright subjects vanish when the fluorescence filter is used.

Additional photos from 24 hours later:

Last update January 31st 2014

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